| Division | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| # | Northeast Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Philadelphia Union II | 5 | 3 | 2 | 0 | 0 | 8:4 | 13 | |
| 2. | Chicago Fire II | 5 | 3 | 1 | 0 | 1 | 9:6 | 11 | |
| 3. | Cincinnati 2 | 5 | 3 | 0 | 1 | 1 | 10:7 | 10 | |
| 4. | New England Revolution II | 3 | 3 | 0 | 0 | 0 | 8:4 | 9 | |
| 5. | Columbus Crew 2 | 5 | 2 | 0 | 0 | 3 | 6:9 | 6 | |
| 6. | New York City II | 4 | 1 | 1 | 0 | 2 | 8:7 | 5 | |
| 7. | Toronto FC II | 3 | 1 | 0 | 2 | 0 | 4:3 | 5 | |
| 8. | New York Red Bulls 2 | 3 | 0 | 2 | 0 | 1 | 8:10 | 4 | |
| # | Southeast Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Chattanooga | 5 | 3 | 2 | 0 | 0 | 14:5 | 13 | |
| 2. | Crown Legacy | 5 | 2 | 1 | 1 | 1 | 10:8 | 9 | |
| 3. | Atlanta United 2 | 7 | 2 | 1 | 0 | 4 | 9:14 | 8 | |
| 4. | Inter Miami II | 4 | 2 | 0 | 0 | 2 | 7:7 | 6 | |
| 5. | Orlando City B | 3 | 1 | 0 | 0 | 2 | 4:5 | 3 | |
| 6. | Huntsville | 5 | 0 | 0 | 2 | 3 | 5:8 | 2 | |
| 7. | Carolina Core | 0 | 0 | 0 | 0 | 0 | 0:0 | 0 | |
| # | Pacific Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Los Angeles FC II | 5 | 4 | 0 | 0 | 1 | 14:8 | 12 | |
| 2. | Ventura County | 4 | 3 | 1 | 0 | 0 | 10:5 | 11 | |
| 3. | Tacoma Defiance | 4 | 3 | 1 | 0 | 0 | 9:4 | 11 | |
| 4. | Portland Timbers 2 | 5 | 3 | 0 | 0 | 2 | 12:8 | 9 | |
| 5. | Real Monarchs | 6 | 2 | 1 | 0 | 3 | 6:7 | 8 | |
| 6. | Town FC | 3 | 1 | 1 | 1 | 0 | 2:1 | 6 | |
| 7. | Vancouver Whitecaps 2 | 2 | 1 | 0 | 1 | 0 | 7:5 | 4 | |
| # | Frontier Division | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Sporting Kansas City II | 6 | 3 | 0 | 0 | 3 | 12:9 | 9 | |
| 2. | St. Louis City 2 | 5 | 2 | 1 | 1 | 1 | 7:5 | 9 | |
| 3. | North Texas | 4 | 1 | 2 | 1 | 0 | 5:4 | 8 | |
| 4. | Minnesota 2 | 2 | 1 | 0 | 0 | 1 | 2:2 | 3 | |
| 5. | Austin FC II | 4 | 0 | 1 | 1 | 2 | 5:8 | 3 | |
| 6. | Colorado Rapids 2 | 4 | 0 | 0 | 1 | 3 | 5:10 | 1 | |
| 7. | Houston Dynamo 2 | 2 | 0 | 0 | 0 | 2 | 1:3 | 0 | |
| # | Eastern Conference | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Chattanooga | 5 | 3 | 2 | 0 | 0 | 14:5 | 13 | |
| 2. | Philadelphia Union II | 5 | 3 | 2 | 0 | 0 | 8:4 | 13 | |
| 3. | Chicago Fire II | 5 | 3 | 1 | 0 | 1 | 9:6 | 11 | |
| 4. | Cincinnati 2 | 5 | 3 | 0 | 1 | 1 | 10:7 | 10 | |
| 5. | New England Revolution II | 3 | 3 | 0 | 0 | 0 | 8:4 | 9 | |
| 6. | Crown Legacy | 5 | 2 | 1 | 1 | 1 | 10:8 | 9 | |
| 7. | Atlanta United 2 | 7 | 2 | 1 | 0 | 4 | 9:14 | 8 | |
| 8. | Inter Miami II | 4 | 2 | 0 | 0 | 2 | 7:7 | 6 | |
| 9. | Columbus Crew 2 | 5 | 2 | 0 | 0 | 3 | 6:9 | 6 | |
| 10. | New York City II | 4 | 1 | 1 | 0 | 2 | 8:7 | 5 | |
| 11. | Toronto FC II | 3 | 1 | 0 | 2 | 0 | 4:3 | 5 | |
| 12. | New York Red Bulls 2 | 3 | 0 | 2 | 0 | 1 | 8:10 | 4 | |
| 13. | Orlando City B | 3 | 1 | 0 | 0 | 2 | 4:5 | 3 | |
| 14. | Huntsville | 5 | 0 | 0 | 2 | 3 | 5:8 | 2 | |
| 15. | Carolina Core | 0 | 0 | 0 | 0 | 0 | 0:0 | 0 | |
| # | Western Conference | MP | W | WP | LP | L | G | Pts | Form |
| 1. | Los Angeles FC II | 5 | 4 | 0 | 0 | 1 | 14:8 | 12 | |
| 2. | Ventura County | 4 | 3 | 1 | 0 | 0 | 10:5 | 11 | |
| 3. | Tacoma Defiance | 4 | 3 | 1 | 0 | 0 | 9:4 | 11 | |
| 4. | Portland Timbers 2 | 5 | 3 | 0 | 0 | 2 | 12:8 | 9 | |
| 5. | Sporting Kansas City II | 6 | 3 | 0 | 0 | 3 | 12:9 | 9 | |
| 6. | St. Louis City 2 | 5 | 2 | 1 | 1 | 1 | 7:5 | 9 | |
| 7. | North Texas | 4 | 1 | 2 | 1 | 0 | 5:4 | 8 | |
| 8. | Real Monarchs | 6 | 2 | 1 | 0 | 3 | 6:7 | 8 | |
| 9. | Town FC | 3 | 1 | 1 | 1 | 0 | 2:1 | 6 | |
| 10. | Vancouver Whitecaps 2 | 2 | 1 | 0 | 1 | 0 | 7:5 | 4 | |
| 11. | Minnesota 2 | 2 | 1 | 0 | 0 | 1 | 2:2 | 3 | |
| 12. | Austin FC II | 4 | 0 | 1 | 1 | 2 | 5:8 | 3 | |
| 13. | Colorado Rapids 2 | 4 | 0 | 0 | 1 | 3 | 5:10 | 1 | |
| 14. | Houston Dynamo 2 | 2 | 0 | 0 | 0 | 2 | 1:3 | 0 | |